| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Coates, Nina A (12)
|
NCNEAL |
33.37 |
34.60 |
1.23 |
| 2 |
Ouellette, Mia G (12)
|
NCGFSC |
35.64 |
35.48 |
-0.16 |
| 3 |
Mitchell, Lily K (11)
|
NCGFSC |
38.94 |
36.79 |
-2.15 |
| 4 |
Rypkema, Ava R (11)
|
NCGFSC |
36.06 |
38.10 |
2.04 |
| 5 |
Mixa, Olivia S (12)
|
NCNEAL |
|
39.27 |
- |
| 6 |
Cauret, Marina D (11)
|
NCGFSC |
42.49 |
40.86 |
-1.63 |
| 7 |
Harper, Gabbrielle E (12)
|
NCGOLD |
40.99 |
41.37 |
0.38 |
| 8 |
Cherry, Taylor A (12)
|
NCAQUA |
|
41.99 |
- |
| 9 |
Freudenberg, Genavieve L (12)
|
NCASTF |
|
45.16 |
- |
| 10 |
Stutts, Caylie E (12)
|
NCRMY |
48.70 |
46.47 |
-2.23 |
| 11 |
Council, Mya M (11)
|
NCRMY |
55.22 |
48.96 |
-6.26 |
|
Chhoeung, Lily V (12)
|
NCASTF |
|
DQ |
- |
|
Shoneman, Sophia G (11)
|
NCAQUA |
|
NS |
- |
|
Keleher, Emma K (11)
|
NCGFSC |
39.16 |
NS |
- |
|
Riggs, Skylar R (12)
|
NCLTNC |
38.80 |
NS |
- |